Comparative evaluation of an organic and an inorganic coagulant in the clarification of vinasse: dose optimization for BOD₅ and turbidity reduction.

Authors

DOI:

https://doi.org/10.18226/25253824.v10.n15.04

Keywords:

Vinasse, clarification, BOD₅ reduction, turbidity removal, polyaluminum chloride

Abstract

Environmental pollution caused by vinasse, a byproduct of alcohol distillation characterized by high organic load and turbidity, presents a significant challenge to the sustainability of distilleries and the quality of aquatic ecosystems. This study aimed to determine the optimal doses of organic and inorganic coagulants for reducing Biochemical Oxygen Demand (BOD₅) and turbidity in vinasse through clarification. The Response Surface Methodology (RSM) was used to evaluate combinations of coagulants and flocculants in a standard coagulation–flocculation system. The main findings were: (1) the combination of the organic coagulant Lipesa 1700 and the flocculant Lipesa 1587A was the most effective, achieving a coefficient of determination (R²) of 96.07% for both BOD₅ and turbidity reduction; (2) optimization identified optimal doses of 2347.47 mg/L of Lipesa 1700 and 20 mg/L of Lipesa 1587A, with adjusted BOD₅ of 17121.0 mg O₂/L and adjusted turbidity of 7084.32 NTU; and (3) the inorganic coagulant Lipesa AC005 showed lower predictive accuracy (R² = 62.24% for BOD₅ and R² = 62.25% for turbidity), indicating lower effectiveness in vinasse clarification. In conclusion, the use of organic coagulants provides greater efficiency in contaminant reduction, although their industrial application should account for operational costs and residue management.

Author Biographies

Walter Manuel Hoyos-Alayo, Universidad Tecnológica del Perú - UTP

Chemical Engineer with a master’s degree in Environmental Engineering and over 10 years of professional experience and 3 years in university teaching. Skilled in: (1) water and effluent treatment, (2) budget management and development of work plans, and (3) knowledge and use of academic virtual platforms for research management (e.g., reference managers, statistical software, Vos Viewer, Bibliometrix, etc.).

Jorge Luis Leiva-Piedra, Universidad Tecnológica del Perú - UTP

Agricultural Engineer with a master’s degree in Crop Protection and over 11 years of experience in university research and 4 years in university teaching. Skilled in: (1) current university regulations, (2) project formulation for competitive funding (INNOVATE - Peru, PNIA, CONCYTEC), (3) budget management and work plan development, (4) implementation and development of new activities based on critical analysis, (5) knowledge and use of academic virtual platforms for research management (e.g., reference managers, statistical software, Eos Viewer, Bibliometrix, etc.), (6) project monitoring and follow-up, (7) preparation of operational plans, (8) applied statistical tools, and (9) management of academic and research laboratories. Current CONCYTEC-registered researcher.

References

Montoya, A., Tejeda, A., Sulbarán-Rangel, B., & Zurita, F. (2023). Treatment of tequila vinasse mixed with domestic wastewater in two types of constructed wetlands. Water Science and Technology, 87(12), 3072-3082. Scopus. https://doi.org/10.2166/wst.2023.189

de Paiva, F. F. G., dos Santos, L. F., Tamashiro, J. R., Silva, L. H. P., Teixeira, S. R., Galvín, A. P., López-Uceda, A., Skowera, K., & Kinoshita, A. (2024). Environmental assessment and durability performance of cement mortar incorporating sugarcane vinasse in replacement of water. Environmental Science and Pollution Research, 31(12), 17690-17705. Scopus. https://doi.org/10.1007/s11356-023-28073-z

Cotta, C. P., Pinto, T. J. S., Yoshii, M. P. C., Silva, L. C. M., Ogura, A. P., Gabriel, G. V. M., Schiesari, L. C., Carmo, J. B., Montagner, C. C., Espíndola, E. L. G., & Moreira, R. A. (2023). Exposure to fipronil, 2,4-D and vinasse influences macroinvertebrate assemblage structure: An experimental mesocosm approach. Science of the Total Environment, 888. Scopus. https://doi.org/10.1016/j.scitotenv.2023.164259

Tejeda, A., Montoya, A., Sulbarán-Rangel, B., & Zurita, F. (2023). Possible Pollution of Surface Water Bodies with Tequila Vinasses. Water (Switzerland), 15(21). Scopus. https://doi.org/10.3390/w15213773

Cajo, Lady, Nizama, L., & Carreño, C. (2011). Efecto de la concentración del inóculo y la melaza como suplemento de la vinaza de destilería para la producción de biomasa de Candida utilis nativa. Scientia Agropecuaria, 2(2), Article 2. https://doi.org/10.17268/sci.agropecu.2011.02.01

Moezzi, S. A., & Khoei, A. J. (2023). Treatment of industrial wastewater of alcohol factories using a particle trap system and their potential for aquaculture using Daphnia (Daphnia pulex) and Zebrafish (Danio rerio) as model bioindicators. International Journal of Aquatic Biology, 11(4), 338-353. Scopus. https://doi.org/10.22034/ijab.v11i4.1936

Tamashiro, J. R., Lima, I. S., Paiva, F. F. G. D., Silva, L. H. P., Oliveira, D. V. M. D., Baffa, O., & Kinoshita, A. (2022). Treatment of Sugarcane Vinasse Using Heterogeneous Photocatalysis with Zinc Oxide Nanoparticles. Sustainability (Switzerland), 14(23). Scopus. https://doi.org/10.3390/su142316052

Ogura, A. P., Moreira, R. A., da Silva, L. C. M., Negro, G. S., Freitas, J. S., da Silva Pinto, T. J., Lopes, L. F. P., Yoshii, M. P. C., Goulart, B. V., Montagner, C. C., & Espíndola, E. L. G. (2022). Irrigation with Water Contaminated by Sugarcane Pesticides and Vinasse Can Inhibit Seed Germination and Crops Initial Growth. Archives of Environmental Contamination and Toxicology, 82(3), 330-340. Scopus. https://doi.org/10.1007/s00244-022-00914-x

Ibrahim, T. H., Oyedele, J. O., Betiku, E., Solomon, B. O., Dahunsi, S. O., & Gidado, R. S. (2022). Biomethane potential and physicochemical characterization of cassava vinasse from ethanol distillery. Current Research in Green and Sustainable Chemistry, 5. Scopus. https://doi.org/10.1016/j.crgsc.2022.100338

El-Dib, F. I., Tawfik, F. M., Eshaq, G., Hefni, H. H. H., & ElMetwally, A. E. (2016). Remediation of distilleries wastewater using chitosan immobilized Bentonite and Bentonite based organoclays. International Journal of Biological Macromolecules, 86, 750-755. Scopus. https://doi.org/10.1016/j.ijbiomac.2016.01.108

Rahman, S., Sabnis, M., Kuusisto, L. M., Sattler, M., & Chen, V. (2018). Models for organics removal from vinasse from ethanol production. Clean Technologies and Environmental Policy, 20(4), 803-812. Scopus. https://doi.org/10.1007/s10098-018-1496-4

Ministerio de la Producción. (2022). Anuario estadístico industrial, mipyme y comercio interno 2022. https://ogeiee.produce.gob.pe/index.php/en/shortcode/oee-documentos-publicaciones/publicaciones-anuales/item/1125-anuario-estadistico-industrial-mipyme-y-comercio-interno-2022

Budianto, G. P. I., Wibowo, Y. M., Hadiyanto, H., & Widayat, W. (2024). Zeolite addition in microalgae Chlorella sp. For treatment of bacteria contaminated vinasse. Environmental Quality Management, 33(3), 121-127. Scopus. https://doi.org/10.1002/tqem.22072

Costa, R. B., Camiloti, P. R., Sabatini, C. A., dos Santos, C. E. D., Lima Gomes, P. C. F., & Adorno, M. Â. T. (2018). Matrix Effect Assessment of an Ion Chromatographic Method to Determine Inorganic Anions in Wastewater. Water, Air, and Soil Pollution, 229(7). Scopus. https://doi.org/10.1007/s11270-018-3863-5

Organismo de Evaluación y Fiscalización Ambiental [OEFA]. (2023). Resolución 157-2023-OEFA-TFA-SE. https://cdn.www.gob.pe/uploads/document/file/4486389/Res%20157-2023-OEFA-TFA-SE.pdf?v=1682612788

Organismo de Evaluación y Fiscalización Ambiental [OEFA]. (2018). Resolución Directoral N° 2219-2018-OEFA/DFAI. https://cdn.www.gob.pe/uploads/document/file/2440598/Resoluci%C3%B3n%20N%C2%B0%202219-2018-OEFA/DFAI.pdf?v=1637092509

Organismo de Evaluación y Fiscalización Ambiental [OEFA]. (2019). RESOLUCIÓN No 007-2019-EFA/TFA-SMEPIM. https://www.oefa.gob.pe/?wpfb_dl=33922

Autoridad Nacional del Agua [ANA]. (2017). Resolución Directoral N° 3076-2017-ANA-AAA-JZ-V. https://www.ana.gob.pe/sites/default/files/normatividad/files/rd-3076-2017.pdf

Autoridad Nacional del Agua [ANA]. (2020). Resolución Directoral-1733-2020-ANA-AAA.JZ-V. https://www.ana.gob.pe/sites/default/files/normatividad/files/07-RD-1733-2020-09.pdf

Monteiro Sobrinho, A. C., Leal, L. C. S. R., Monteiro Neto, J. L. L., Chambarelli, M. C. M. C., & Bittencourt, A. J. (2023). Evaluation in vitro of the virulence of two entomopathogenic heterorhabditid nematodes in the control of Stomoxys calcitrans (Diptera: Muscidae) larvae in byproducts of the sugar and alcohol industry. Revista Brasileira de Parasitologia Veterinaria, 32(2). Scopus. https://doi.org/10.1590/S1984-29612023024

Fukushima, N. A., Palacios-Bereche, M. C., Palacios-Bereche, R., & Nebra, S. A. (2019). Energy analysis of the ethanol industry considering vinasse concentration and incineration. Renewable Energy, 142, 96-109. Scopus. https://doi.org/10.1016/j.renene.2019.04.085

Paz-Pino, O. L., Barba-Ho, L. E., & Marriaga-Cabrales, N. (2014). Vinasse treatment by coupling of electro-dissolution, heterocoagulation and anaerobic digestion. DYNA (Colombia), 81(187), 102-107. Scopus. https://doi.org/10.15446/dyna.v81n187.38922

Guerreiro, L. F., Rodrigues, C. S. D., Duda, R. M., de Oliveira, R. A., Boaventura, R. A. R., & Madeira, L. M. (2016). Treatment of sugarcane vinasse by combination of coagulation/flocculation and Fenton’s oxidation. Journal of Environmental Management, 181, 237-248. Scopus. https://doi.org/10.1016/j.jenvman.2016.06.027

Rodrigues, C. S. D., Neto, A. R., Duda, R. M., de Oliveira, R. A., Boaventura, R. A. R., & Madeira, L. M. (2017). Combination of chemical coagulation, photo-Fenton oxidation and biodegradation for the treatment of vinasse from sugar cane ethanol distillery. Journal of Cleaner Production, 142, 3634-3644. Scopus. https://doi.org/10.1016/j.jclepro.2016.10.104

Lebron, Y. A. R., Moreira, V. R., Furtado, T. P. B., da Silva, S. C., Lange, L. C., & Amaral, M. C. S. (2020). Vinasse treatment using hybrid tannin-based Coagulation-Microfiltration-Nanofiltration processes: Potential energy recovery, technical and economic feasibility assessment. Separation and Purification Technology, 248. Scopus. https://doi.org/10.1016/j.seppur.2020.117152

Sacchi, G. D., Leite, L. S., Reali, M. A. P., Bichara, A., & Seleghim, M. H. R. (2020). Coagulation and Microfiltration Application for Sugarcane Vinasse Clarification. Water, Air, and Soil Pollution, 231(12). Scopus. https://doi.org/10.1007/s11270-020-04944-w

Kee, W.-C., Wong, Y.-S., Ong, S.-A., Lutpi, N. A., Sam, S.-T., Dahalan, F. A., Chai, A., & Eng, K.-M. (2023). Chemical and biological combined treatment for sugarcane vinasse: Selection of parameters and performance studies. Environmental Science and Pollution Research, 30(24), 65364-65378. Scopus. https://doi.org/10.1007/s11356-023-27046-6

Silva, S. C. D., Moravia, M. C. S. A., & Couto, C. F. (2020). COMBINED PROCESS OF ULTRAFILTRATION AND NANOFILTRATION FOR VINASSE TREATMENT WITH AND WITHOUT PRE-COAGULATION. Journal of Water Process Engineering, 36. Scopus. https://doi.org/10.1016/j.jwpe.2020.101326

Syaichurrozi, I., Sarto, S., Sediawan, W. B., & Hidayat, M. (2020). Mechanistic model of electrocoagulation process for treating vinasse waste: Effect of initial pH. Journal of Environmental Chemical Engineering, 8(3). Scopus. https://doi.org/10.1016/j.jece.2020.103756

Prodanović, J. M., Şćiban, M. B., Antov, M. G., & Dodić, J. M. (2011). Comparing the use of common bean extracted natural coagulants with centrifugation in the treatment of distillery wastewaters. Romanian Biotechnological Letters, 16(5), 6638-6647. Scopus.

López-Pérez, L., Pérez-Béz, S. O., & Susial, P. (2001). Study and economic analysis in the treatment of vinasse. Tecnologia del Agua, 21(208), 48-56. Scopus.

Sica, P., Carvalho, R., Beltrame, H., & Baptista, A. S. (2020). Assessment of the Effects of Sugarcane Straw Addition to the Flocculation/Coagulation Process on Vinasse Concentration. Acta Technologica Agriculturae, 23(3), 132-136. Scopus. https://doi.org/10.2478/ata-2020-0021

Castro, L. E. N., Santos, J. V. F., Fagnani, K. C., Alves, H. J., & Colpini, L. M. S. (2019). Evaluation of the effect of different treatment methods on sugarcane vinasse remediation. Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes, 54(9), 791-800. Scopus. https://doi.org/10.1080/03601234.2019.1669981

Andersen, S. J., Candry, P., Basadre, T., Khor, W. C., Roume, H., Hernandez-Sanabria, E., Coma, M., & Rabaey, K. (2015). Electrolytic extraction drives volatile fatty acid chain elongation through lactic acid and replaces chemical pH control in thin stillage fermentation. Biotechnology for Biofuels, 8(1). Scopus. https://doi.org/10.1186/s13068-015-0396-7

Rodrigues, I. J., Fuess, L. T., Biondo, L., Santesso, C. A., & Garcia, M. L. (2014). Coagulation-flocculation of anaerobically treated sugarcane stillage. Desalination and Water Treatment, 52(22-24), 4111-4121. Scopus. https://doi.org/10.1080/19443994.2013.801785

Lin, C.-Y., Chiang, C.-C., Nguyen, T. M. L., & Lay, C.-H. (2017). Continuous biohydrogen production from coagulation-pretreated textile desizing wastewater. International Journal of Hydrogen Energy, 42(49), 29159-29165. Scopus. https://doi.org/10.1016/j.ijhydene.2017.10.012

Rennola, L., Yépez, C., Bullón, J., & Salazar, F. (2007). Treatment of destillery wastewaters using coagulants and membranes. Revista Tecnica de la Facultad de Ingenieria Universidad del Zulia, 30(SPEC. ISSUE). Scopus.

Alkan-Ozkaynak, A., Karthikeyan, K. G., & Roa-Espinosa, A. (2010). Reducing phosphorus concentration in animal feed coproducts from the corn distilling industry. Transactions of the ASABE, 53(4), 1287-1294. Scopus.

Karchiyappan, T., Delcolle, R. D., Goncalves, G. L., Vareschini, D. T., & Gimenes, M. L. (2019). Treatment of vinasse liquid from sugarcane industry using electro-coagulation/flocculation followed by ultra filtration. Polish Journal of Chemical Technology, 21(4), 40-47. Scopus. https://doi.org/10.2478/pjct-2019-0037

Formann, S., Hahn, A., Janke, L., Stinner, W., Sträuber, H., Logroño, W., & Nikolausz, M. (2020). Beyond Sugar and Ethanol Production: Value Generation Opportunities Through Sugarcane Residues. Frontiers in Energy Research, 8. Scopus. https://doi.org/10.3389/fenrg.2020.579577

Tse, T. J., Shen, J., Shim, Y. Y., & Reaney, M. J. T. (2020). Changes in Bacterial Populations and Their Metabolism over 90 Sequential Cultures on Wheat-Based Thin Stillage. Journal of Agricultural and Food Chemistry, 68(16), 4717-4729. Scopus. https://doi.org/10.1021/acs.jafc.9b07414

Ratanapariyanuch, K., Shim, Y. Y., Emami, S., & Reaney, M. J. T. (2017). Industrial clarification of wheat-based distillers’ solubles and thin stillage. Industrial Crops and Products, 109, 828-835. Scopus. https://doi.org/10.1016/j.indcrop.2017.09.029

Padilha, R. S., Ferrari-Lima, A. M., Seixas, F. L., Batistela, V. R., Fávaro, S. L., Hioka, N., & Fernandes-Machado, N. R. C. (2013). Evaluation of ZnO catalyst supported on zeolite NaA in the photocatalytic degradation of vinasse pretreated by coagulation/flocculation. Chemical Engineering Transactions, 32, 823-828. Scopus. https://doi.org/10.3303/CET1332138

Nunes, N. S. P., de Almeida, J. M. O., Fonseca, G. G., & de Carvalho, E. M. (2022). Clarification of sugarcane (Saccharum officinarum) vinasse for microalgae cultivation. Bioresource Technology Reports, 19. Scopus. https://doi.org/10.1016/j.biteb.2022.101125

Montaño Saavedra, M. D., Cárdenas Concha, V. O., & Bastos, R. G. (2024). Electrocoagulation treatment of sugarcane vinasse: Operating parameters and cost analysis by response surface methodology. Journal of Cleaner Production, 448. Scopus. https://doi.org/10.1016/j.jclepro.2024.141597

Devesa-Rey, R., Bustos, G., Cruz, J. M., & Moldes, A. B. (2011). Optimisation of entrapped activated carbon conditions to remove coloured compounds from winery wastewaters. Bioresource Technology, 102(11), 6437-6442. Scopus. https://doi.org/10.1016/j.biortech.2011.03.072

Ratanapariyanuch, K., Shim, Y. Y., Wiens, D. J., & Reaney, M. J. T. (2018). Grain Thin Stillage Protein Utilization: A Review. JAOCS, Journal of the American Oil Chemists’ Society, 95(8), 933-942. Scopus. https://doi.org/10.1002/aocs.12056

Menkhaus, T. J., Anderson, J., Lane, S., & Waddell, E. (2010). Polyelectrolyte flocculation of grain stillage for improved clarification and water recovery within bioethanol production facilities. Bioresource Technology, 101(7), 2280-2286. Scopus. https://doi.org/10.1016/j.biortech.2009.11.017

de Souza, R. P., Girardi, F., Santana, V. S., Fernandes-Machado, N. R. C., & Gimenes, M. L. (2013). Vinasse treatment using a vegetable-tannin coagulant and photocatalysis. Acta Scientiarum - Technology, 35(1), 89-95. Scopus. https://doi.org/10.4025/actascitechnol.v35i1.11011

de Souza, R. P., Ferrari-Lima, A. M., Pezoti, O., Santana, V. S., Gimenes, M. L., & Fernandes-Machado, N. R. C. (2016). Photodegradation of sugarcane vinasse: Evaluation of the effect of vinasse pre-treatment and the crystalline phase of TiO2. Acta Scientiarum - Technology, 38(2), 217-226. Scopus. https://doi.org/10.4025/actascitechnol.v28i2.27440

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Published

2026-03-16

How to Cite

Hoyos-Alayo, W. M., & Leiva-Piedra, J. L. (2026). Comparative evaluation of an organic and an inorganic coagulant in the clarification of vinasse: dose optimization for BOD₅ and turbidity reduction. Revista Interdisciplinar De Ciência Aplicada, 10(15), 1–12. https://doi.org/10.18226/25253824.v10.n15.04