Inhibitory action of fermented Cypress extracts on Colletotrichum fungi isolated from Acca sellowiana
DOI:
https://doi.org/10.18226/25253824.v10.n15.10Palavras-chave:
Regenerative agriculture, Botanical fermentations, Food securityResumo
Este estudo teve como objetivo avaliar a eficiência de extratos botânicos fermentados de Chamaecyparis pisifera, Platycladus orientalis e Cupressus sempervirens no controle in vitro de C. fructicola, C. theobromicola e C. pseudoacutatum, isolados de A. sellowiana. Os compostos fenólicos dos diferentes extratos botânicos fermentados foram analisados por cromatografia líquida de alta eficiência (HPLC). Na avaliação in vitro, os diferentes extratos botânicos foram incorporados ao meio PDA nas concentrações de 0%, 10%, 20% e 40% (v/v). O efeito sobre o crescimento micelial dos patógenos foi avaliado após 14 dias, e a percentagem de inibição do crescimento foi determinada. O teste in vivo foi realizado apenas com os melhores resultados obtidos nos testes in vitro, ou seja, com o extrato fermentado de C. pisifera contra os fitopatógenos C. fructicola e C. theobromicola. Nos testes com C. fructicola, o extrato de C. pisifera apresentou 50% de inibição na concentração de 40% (v/v). Para C. theobromicola, o extrato de C. pisifera inibiu 48% na mesma concentração, enquanto os outros extratos não apresentaram atividade antifúngica. Nos ensaios com C. pseudoacutatum, os extratos fermentados de C. pisifera, C. sempervirens e P. orientalis não exibiram efeito antifúngico. Nos testes in vivo, os tratamentos T1 a T4 foram eficazes na redução da incidência e severidade da antracnose, com os melhores resultados observados em T2 (C. pisifera com C. fructicola) e T4 (C. pisifera com C. theobromicola), reduzindo a incidência em 59% e 54%, respectivamente. Os dados sugerem que o extrato fermentado de C. pisifera tem potencial como agente protetor contra a antracnose.
Referências
[1] Amarante, CVT & Santos, KLS (2013). Mountain guava (Acca sellowiana). Brazilian Journal of Fruit Growing, 33(1), 001334.https://www.scielo.br/j/rbf/a/KP3cXy6XXRvpQWHg4BnjQgB
[2] Gutz, T., Neves, LO, Vicentin, E., Melo, LC, & Goede, A. (2015). Assessment of the adaptability of the mountain guava crop in Agroforestry System (SAF) in the Alto Vale do Itajaí region/SC. In: VIII National Exhibition of Interdisciplinary Scientific and Technological Initiation, Santa Rosa do Sul. Proceedings of the VIII MICTI.
[3] Santos, KL (2009). Cultural, genetic and phenotypic diversity of the mountain guava (Acca sellowiana): implications for the domestication of the species (Doctoral Thesis in Plant Genetic Resources, Federal University of Santa Catarina, Florianópolis).
[4] Shin, HY, Kim, SM, Lee, JH, & Lim, ST (2019). Solid-state fermentation of black rice bran with Aspergillus awamori and Aspergillus oryzae: Effects on phenolic acid composition and antioxidant activity of bran extracts. Food Chemistry, 272, 235-241. https://doi.org/10.1016/j.foodchem.2018.07.174
[5] Dean, R., Van Kan, JAL, Pretorius, ZA, Hammond-Kosack, KE, Di Pietro, A., Spanu, P.D., Rudd, J.J., Dickman, M., Kahmann, R., Ellis, J., & Foster, G.D. (2012). The Top 10 fungal pathogens in molecular plant pathology. Molecular Plant Pathology, 13(4), 414–430. https://doi.org/10.1111/j.1364-3703.2011.00783.
[6] Dey, T. B., Chakraborty, S., Jain, K. K., Sharma, A., & Kuhad, R. C. (2016). Antioxidant phenolics and their microbial production by submerged and solid state fermentation process: A review. Trends in Food Science & Technology, 53, 60-74. https://doi.org/10.1016/j.tifs.2016.04.007
[7] Ciotta, MN, Arioli, CJ, Pinto, FAMF, Santos, KL, Araujo, L., & Pasa, M. da S. (Orgs.). (2018). The culture of mountain guava. 1st ed. Florianopolis: Epagri.
[8] Ferreira-Saab, M., Formey, D., Torres, M., Aragón, W., Padilla, EA, Tromas, A., Sohlenkamp, C., Schwan-Estrada, KRF, & Serrano, M. (2018). Compounds released by the biocontrol yeast Hanseniaspora opuntiae protect plants against Corynespora cassiicola and Botrytis cinerea. Frontiers in Microbiology, 9(1596), 1596-1608. https://doi.org/10.3389/fmicb.2018.01596
[9] Alengebawy, A., Abdelkhalek, S. T., Qureshi, S. R., & Wang, M.-Q. (2021). Heavy Metals and Pesticides Toxicity in Agricultural Soil and Plants: Ecological Risks and Human Health Implications. Toxics, 9(3), 42. https://doi.org/10.3390/toxics9030042
[11] Rozwalka, L.C.; Alves, E.; Amaral, D.C. Ultrastructural study of Colletotrichum gloeosporioides and Colletotrichum musae conidia treated with essential oils. Interciencia, 35, 912–915 (2010)
[12] Da Cruz Cabral, L., Fernández Pinto, V., & Patriarca, A. (2013). Application of plant derived compounds to control fungal spoilage and mycotoxin production in foods. International Journal of Food Microbiology, 166(1), 1–14. https://doi.org/10.1016/j.ijfoodmicro.2013.05.026
[13] Hussain, A., Bose, S., Wang, J. H., Yadav, M. K., Mahajan, G. B., & Kim, H. (2016). Fermentation, a feasible strategy for enhancing bioactivity of herbal medicines. Food Research International, 81, 1-16. https://doi.org/10.1016/j.foodres.2015.12.026
[14] Mac Loughlin, T. M., Peluso, L., & Marino, D. G. (2017). Pesticide impact study in the peri-urban horticultural area of Gran La Plata, Argentina. Science of the Total Environment, 598, 572-580. https://doi.org/10.1016/j.scitotenv.2017.04.116
[15] Sartori. VC, & Venturin. L. (2016). Alternative technologies for strengthening family farming in Serra Gaúcha. (2nd ed.). Caxias do Sul, RS. Educs.
[16] Pansera, MR.; Silvestre, WP; Touguinha, LBA; Sartori, VC. Antifungal and Antioxidant Activity of Cupressus sempervirens and Cupressus lusitanica Botanical Fermentates on Colletotrichum fructicola: In Vitro and In Vivo Evaluation. Journal of Social and Environmental Management, v. 17, no. 10, p. e04061, 2023.
[17] Costa, B., Pansera, M.R., & Sartori, V.C. (2021). Evaluation of fermented botanicals in the control of Colletotrichum fructicola isolated from Acca sellowiana. Research, Society and Development, 10(5), e48910515116. https://doi.org/10.33448/rsd-v10i5.15116
[18] Agostini, F., Michelon, FM, Gomes, V.A.A., Bertolazzi, S., Schwambach, J., & Moura, S. (2017). Optimization of a UV-HPLC method for analysis of phenolic compounds in Myrcia oblongata DC., Passiflora caerulea L., and Equisetum giganteum L. Scientia Chromatographica, 9(3), 180–193.
[19] Morelli, LLL (2010). Evaluation of major phenolic compounds in grape jelly produced with the IAC-138-22 (maximum) variety. Master's thesis. State University of Campinas. Campinas.
[20] Badawy MEI; Rabea, EI Preparation and antimicrobial activity of O-(benzoyl) chitosan derivatives against some plant pathogens. African Journal of Microbiology Research, 7, 2259–2268 10.5897/AJMR12.1185 (2022)
[21] Songwattana, P., Boonchuen, P., Piromyou, P., Wongdee, J., Greetatorn, T., Inthaisong, S., et al. (2023). Insights into antifungal mechanisms of Bacillus velezensis S141 against Cercospora leaf spot in mungbean (V. radiata). Microbes Environment 38:ME22079. doi: 10.1264/jsme2.ME22079
[22] Chankaew, S.; Somta, P.; Sorajjapinun, W.; Srinives, P. Quantitative trait loci mapping of cercospora leaf spot resistance in mung bean, Vigna radiata (L.) Wilczek. Molecular Breeding, 28 (2011), pp. 255-264
[23] Betts, J., Wareham, D., Haswell, S., & Kelly, S. (2013). Antifungal synergy of theaflavin and epicatechin combinations against Candida albicans. Journal of Microbiology and Biotechnology, 23(9), 1322- 1326. https://doi.org/10.4014/jmb.1303.03010
[24] Triaca, T., Pansera, M.R., Andreolla, M.L., Venturin, L., & Sartori, V.C. (2018). In vivo evaluation of the fermented botanical Ilex paraguariensis against the fungus Sclerotinia sclerotiorum in crisp lettuce cultivation. Brazilian Journal of Applied Technology for Agricultural Science/Applied Research & Agrotechnology, 11(1), 51-58. 10.5935/ PAeT.V11. N1.06
[25] Ullah, C., Unsicker, S. B., Fellenberg, C., Constabel, C. P., Schmidt, A., Gershenzon, J., & Hammerbacher, A. (2017). Flavan-3-ols are an effective chemical defense against rust infection. Plant Physiology, 175(4), 1560-1578. https://doi.org/10.1104/pp.17.00842
[26] Zhang, M., Wang, D., Gao, X., Yue, Z., & Zhou, H. (2020). Exogenous caffeic acid and epicatechin enhance resistance against Botrytis cinerea through activation of the phenylpropanoid pathway in apples. Scientia horticulturae, 268, 109348. https://doi.org/10.1016/j.scienta.2020.109348
[27] Borges, S., Alkassab, A. T., Collison, E., Hinarejos, S., Jones, B., McVey, E., et al. (2023). Overview of the testing and assessment of effects of microbial pesticides on bees: strengths, challenges, and perspectives. Apidologie 52, 1256–1277. doi: 10.1007/s13592-021-00900-7
[28] Sardi, JDCO, Freires, IA, Lazarini, JG, Infante, J., de Alencar, SM, & Rosalen, PL (2017). Unexplored endemic fruit species from Brazil: Antibiofilm properties, insights into mode of action, and systemic toxicity of four Eugenia spp. Microbial Pathogenesis, 105, 280-287. https://doi.org/10.1016/j.micpath.2017.02.044
[29] Girardi, F. A., Tonial, F., Chini, S. O., Sobottka, A. M., Scheffer-Basso, S. M., & Bertol, C. D. (2014). Phytochemical profile and antimicrobial properties of Lotus spp. (Fabaceae). Annals of the Brazilian Academy of Sciences, 86, 1295–1302. https://doi.org/10.1590/0001-3765201420130220
[30] Castro, MR, Victoria, F. N., Oliveira, D. H., Jacob, R. G., Savegnago, L., & Alves, D. (2015). Essential oil of Psidium cattleianum leaves: Antioxidant and antifungal activity. Pharmaceutical Biology, 53(2), 242-250. https://doi.org/10.3109/13880209.2014.914231
[31] Duarte, DHJD; Morais, ES; Siqueira, ES; Nobre, FC; Nobre, LHN Perception and tolerance to the risk of family agricultural managers. Journal of Social and Environmental Management, v. 17, n. 2, e3193, 2023
[32] Cox-Georgian, D., Ramadoss, N., Dona, C., & Basu, C. (2019). Therapeutic and Medicinal Uses of Terpenes. Medicinal Plants: From Farm to Pharmacy, 333–359. https://doi.org/10.1007/978-3-030-31269-5_15
[33] Haapakorva, E., Holmbom, T., & von Wright, A. (2018). Novel aqueous oil-in-water emulsions containing extracts of natural coniferous resins are strongly antimicrobial against enterobacteria, staphylococci, and yeasts, as well as on bacterial biofilms. Journal of Applied Microbiology, 124(1), 136-143. https://doi.org/10.1111/jam.13640
[34] Tsao, NW, Lin, YC, Tseng, YH, Chien, SC, & Wang, SY (2022). Composition analysis of exudates produced by conifers grown in Taiwan and their antifungal activity. Journal of Wood Science, 68, 46. https://doi.org/10.1186/s10086-022-02056-z
[35] Karchesy, JJ, Kelsey, RG, & González-Hernández, MP (2018). Yellow-cedar, Callitropsis (Chamaecyparis) nootkatensis, secondary metabolites, biological activities, and chemical ecology. Journal of Chemical Ecology, 44, 510-524. https://doi.org/10.1007/s10886-018-0956-y
[36] Ignea, C., Athanasakoglou, A., Andreadelli, A., Apostolaki, M., Iakovides, M., Stephanou, E. G., & Kampranis, S. C. (2017). Overcoming the plasticity of plant specialized metabolism for selective diterpene production in yeast. Scientific Reports, 7(8855), 1-11. https://doi.org/10.1038/s41598-017-09592-5
[37] Lasram, S., Zemni, H., Hamdi, Z., Chenenaoui, S., Houissa, H., Tounsi, M. S., & Ghorbel, A. (2019). Antifungal and antiaflatoxinogenic activities of Carum carvi L., Coriandrum sativum L. seed essential oils and their major terpene component against Aspergillus flavus. Industrial Crops and Products, 134, 11-18. https://doi.org/10.1016/j.indcrop.2019.03.037
[38] Pontin, M., Bottini, R., Burba, J. L., & Piccoli, P. (2015). Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum. Phytochemistry, 115, 152-160. https://doi.org/10.1016/j.phytochem.2015.02.003
[39] Torres-León, C., Ramírez-Guzmán, N., Ascacio-Valdes, J., Serna-Cock, L., dos Santos Correia, MT, Contreras-Esquivel, JC, & Aguilar, CN (2019). Solid-state fermentation with Aspergillus niger to enhance the phenolic contents and antioxidative activity of Mexican mango seed: A promising source of natural antioxidants. LWT - Food Science and Technology, 112, 108236. https://doi.org/10.1016/j.lwt.2019.06.003
[40] Azzaz, NA; Hamed, SS; Kenawy, TA Chemical Studies on Cypress Leaves (Cupressus sempervirens) and Their Activity as Antimicrobial Agents. Al-Azhar Journal of Agricultural Research, 2019, 44, 100–109.
[41] Galovičová, L.; Čmiková, N.; Schwarzová, M.; Vukic, MD; Vukovic, NL; Kowalczewski, P.Ł.; Bakay, L.; Kluz, MI; Puchalski, C.; Obradovic, AD; et al. Biological Activity of Cupressus sempervirens. Essential Oil. Plants, 2023, 12, 1097.
[42] Al-Rajhi AMH, Bakri MM, Qanash H, Alzahrani HY, Halawani H, Algaydi MA, Abdelghany TM. Antimicrobial, Antidiabetic, Antioxidant, and Anticoagulant Activities of Cupressus sempervirens In Vitro and In Silico. Molecules. 2023 Nov 2; 28(21):7402. doi: 10.3390/molecules28217402
[43] Bae S, Han JW, Dang QL, Kim H, Choi GJ. Plant Disease Control Efficacy of Platycladus orientalis and Its Antifungal Compounds. Plants. 2021 Jul 21;10(8):1496. doi: 10.3390/plants10081496. PMID: 34451541; PMCID: PMC8400505.
[44] Barros, DB, Nascimento, NS, Sousa, AP, Barros, AV, Borges, YWB, Silva, WMN, Motta, ABS, Pinto, JEL, Sampaio, MGV, Barbosa, MFS, Fonseca, MC, Silva, LA, Lima, LO, Borges, MGSA, Oliveira, MBM, Correia, MTS, Castellano, LRC, Guerra, FQS, & Silva, MV (2023). Antifungal activity of terpenes isolated from the Brazilian Caatinga: a review. Brazilian Journal of Biology, 83, e270966. https://doi.org/10.1590/1519-6984.270966
[45] Xavier, V., Spréa, R., Finimundy, TC, Heleno, SA, Amaral, JS, Barros, L., & Ferreira, IC (2023). Terpenes. In: Natural Secondary Metabolites: From Nature, Through Science, to Industry (pp. 107-156). Cham: Springer International Publishing.
[46] Samet, M.; Charfeddine, M.; Kamoun, L.; Nouri-Ellouze, O.; Gargouri-Bouzid, R. Effect of compost tea containing phosphogypsum on potato plant growth and protection against Fusarium solani infection. Environmental Science and Pollution Research, 25, 18921- 18937, 2018.
[47] Tariq, S.; Wani, S.; Rasool, W.; Bhat, M.A.; A. Prabhakar, A.H. Shalla, M.A. Rather and M.A. Rather, A comprehensive review of the antibacterial, antifungal, and antiviral potential of essential oils and their chemical constituents against drug-resistant microbial pathogens. Microbial Pathogenesis, 134, 103580 (2019). 10.1016/j.micpath.2019.103580
[48] Bonanomi, G.; Gaglione, AS; Cesarano, G.; Sarker, CT; Pascale, M.; Scala, F.; Zoina, A. Frequent Applications of Organic Matter to Agricultural Soil Increase Fungistasis. Pedosphere, v. 27, no. 1, p.86-95, 2017. https://doi.org/10.1016/S1002-0160(17)60298-4
[49] Cha, JY; Han, S.; Hong, H.J.; Cho, H.; Kim, D.; Kwon, Y.; Kwon, S.K.; Crusemann, M.; Bok, L.Y.; Kim, J.F.; Giaever, G.; Nislow, C.; Moore, B.S.; Thomashow, L. S.; Weller, DM; Kwak, YS Microbial and biochemical basis of a Fusarium wilt-suppressive soil. The ISME Journal, vol. 10, 119, 2016.
[50] Pulz, AL, Crusciol, CAC, Lemos, LB, & Soratto, RP (2008). Influence of silicate and limestone on nutrition, productivity, and quality of potato under water deficit. Brazilian Journal of Soil Science, 32 (4), 1651–1659. https://doi.org/10.1590/s0100-06832008000400030
[51] Santos, GR, Rodrigues, AC, Bonifacio, A., Chagas Junior, AF, & Tschoeke, PH (2014). Anthracnose severity in sorghum leaves subjected to increasing doses of silicon. Ciência Agronomica, 45 (2), 403–408. https://doi.org/10.1590/s1806-66902014000200023
[52] Voigt, CA Callose-modified resistance to pathogenic intruders in plant defense-related papillae. Frontiers in Plant Science, 5:168, 2014
[53] Oerke, EC; U Steiner, HW Dehne, M Lindenthal, Thermal imaging of cucumber leaves affected by downy mildew and environmental conditions, Journal of Experimental Botany, Volume 57, Issue 9, June 2006, Pages 2121–2132, https://doi.org/10.1093/jxb/erj170
[54] Nogueira Júnior AF, Ribeiro RV, Appezzato-da-Glória B, Soares MKM, Rasera JB, Amorim L. Phakopsora euvitis Causes Unusual Damage to Leaves and Modifies Carbohydrate Metabolism in Grapevine. Front Plant Sci 2017 Sep 26;8:1675. doi: 10.3389/fpls.2017.01675. PMID: 29018470; PMCID: PMC5623187.
[55] Keller, M. Environmental constraints and stress physiology. In: KELLER, M. The Science of Grapevines: Anatomy and Physiology. 2nd ed. Cambridge: Academic Press, 2010. Chapter 7, p. 267-341. Doi: https://doi.org/10.1016/C2013-0-06797-7.
Downloads
Publicado
Como Citar
Edição
Seção
Licença
Copyright (c) 2026 Laura Araújo Ceccato, Marcia Regina Pansera, Vitória Maria de Miranda Zuccoloto, Vinícius Spolaor Fantinel, Wendel Paulo Silvestre, Valdirene Camatti Sartori
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License. Autores mantém os direitos autorais e concedem à revista o direito de primeira publicação, com o trabalho simultaneamente licenciado sob a Creative Commons Attribution License que permitindo o compartilhamento do trabalho com reconhecimento da autoria do trabalho e publicação inicial nesta revista.
